jump to the content without reading the navigation

Hamaguchi Lab.

Spectrophysical Chemistry bridges chemistry and biology


content start

785nm Near-Infrared-Excited Raman Microspectroscopic System

Near-Infrared-Excited Raman Microspectroscopy

Raman Microspectroscopy
Vibrational spectra reflect molecular structures
suitable for in vivo measurement
sub-µm spacial resolution
disadvantage: interference from fluorescence → Near-infrared excitation enables us to eliminate the interference from fluorescence
Molecular labeling for protein detection
By using a label molecule, which has large NIR Raman scattering cross section, sensitivity of Raman detection is improved. (Fig. 1)
Fig. 1

Fig. 1. Molecular labeling

Experimental Set-Up

By using a Ti:Sapphire laser, high laser power and wavelength tunability become available.

Fig. 2

Fig. 2. Apparatus

Fig. 3

Fig. 3. Setup

Molecular Labeling

Dabsyl (Fig.4) labeling increases the detection sensitivity of bovine serum albumin (BSA) by a factor of ten. Raman mapping shows good contrast image of BSA location by using the BSA labeling. (Fig. 5)

Fig. 4

Fig. 4. Label molecule: dabsyl chloride

Fig. 5

Fig. 5. Raman mapping image

Copyright Hamaguchi Lab. All rights reserved.