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Hamaguchi Lab.

Spectrophysical Chemistry bridges chemistry and biology


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Hyper-Raman Microspectroscopic System

Hyper-Raman (HR) Microspectroscopic System

Hyper Raman scattering
Although signal light intensity is very weak, HR spectra provide unique spectroscopic information.
  • Vibrational selection rule
    • All IR active modes are HR active
       → Posssible alternative to IR microscopy
    • IR/Raman inactive modes can be HR active
  • Resonance HR scattering
    • Information on two-photon allowed electronic states can be obtained.
Fig. 1

Fig. 1. Energy Diagram

Experimental Setup

Spectral resolution: ∼10cm-1, Excitation power: <8mW


Fig. 2. Setup


Fig. 3. Apparatus


IR active vibrational imaging with sub-micrometer spatial resolution is achieved

Fig. 4

Fig. 4. IR active vibrational image of all-trans-β-carotene microcrystall

A new phenomenon was discovered!! Solvent HR signal enhancement by solute molecules: Molecular Near-field Effect.

Fig. 5

Fig. 5. HR spectrum of all-trans-β-carotene in solution showed enhanced solvent HR signals which correspond very well to strong IR bands of solvent.

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